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Can you do a piece on the fusogenicity of the Furin Cleavage Site and spike triggered syncytia? https://www.oncotarget.com/article/28088/text/

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Maples recently posted this, which refers to the FCS:

Structural analysis of SARS-CoV-2 genome and predictions of the human interactome

Andrea Vandelli et al. Nucleic Acids Res. 2020.

https://pubmed.ncbi.nlm.nih.gov/33068416/

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Apr 15, 2023Ā·edited Apr 15, 2023Author

Great suggestion. FCS fusogenicity has been covered in detail by others recently, happy to recap, but from my notes:

Human immunodeficiency virus type-1 envelope glycoprotein gp120 induces expression of fusion regulatory protein (FRP)-1/CD98 on CD4+ T cells: a possible regulatory mechanism of HIV-induced syncytium formation

S Suga et al. Med Microbiol Immunol. 1997

https://pubmed.ncbi.nlm.nih.gov/9138296/

Gp120>CD98>CD147>syncytial formation

And:

The SARS-CoV-2 Spike protein disrupts human cardiac pericytes function through CD147 receptor-mediated signalling: a potential non-infective mechanism of COVID-19 microvascular disease

https://doorlesscarp953.substack.com/p/the-sars-cov-2-spike-protein-disrupts

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I think the whole Homokaryon and heterokaryon thing could be the reason for what is commonly referred to Long COVID, apart from a lot of other people suddenly dying or developing weird symptoms or getting cancer shortly after vax shot.

Just think about it: Spike on cell membrane can fuse with other ACE2 presenting cells. In case of homocaryons, you could end up with cells fusing which are in different stages of their lifecycle. The resulting multinucleated cell could survive that fusion. What "functions" would it have? What would happen if those multinucleated cells have daughter cells? And then add all kinds of different cells to the mix, when you do not need ACE2 for syncytia formation but the FCS could also pull in other cells... Just think about exosomes floating around your bloodstream to every corner of your body ready to fuse cells together that do not belong fused together. What kind of chimeras would you get? What would they do? I think the possibilities are endless. And I think it gets worse when you add the IgG1-> IgG4 shift into the mix! Have those exosome float around even longer, not being cleared by the immune system... What happens when vaxxed people are getting reinfected over and over...

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Apr 16, 2023Ā·edited Apr 16, 2023Author

I personally think gp120 is behind much of LC: T cell apoptosis and neurotoxicity.

But syncytia formation caused by S is also a nightmare. Block the FCS you block syncytia formation.

And we already know that what they call IFITMs in the vaxxed are being suppressed via lncRNA interactions.

Syncytia formation by SARS-CoV-2-infected cells

...Expression of S without any other viral proteins triggers syncytia formation. Interferon-induced transmembrane proteins (IFITMs), a family of restriction factors that block the entry of many viruses, inhibit S-mediated fusion, with IFITM1 being more active than IFITM2 and IFITM3. On the contrary, the TMPRSS2 serine protease, which is known to enhance infectivity of cell-free virions, processes both S and ACE2 and increases syncytia formation by accelerating the fusion process. TMPRSS2 thwarts the antiviral effect of IFITMs. Our results show that SARS-CoV-2 pathological effects are modulated by cellular proteins that either inhibit or facilitate syncytia formation.

https://pubmed.ncbi.nlm.nih.gov/33051876/

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I already saw this one. But yeah the scary stuff is when you have insertions or syncytia that survive. After that it can only get worse....

Syncytia also leads to Lymphopenia...https://www.nature.com/articles/s41418-021-00795-y shown in autopsies of people that died from COVID pneumonia. They found cell-in-cell structures with Clusters of Differentiation belonging to Lymphocytes.

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Thanks DC.

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Another important question now becomes what to do with blood donations from people who were jabbed with LNP/mRNA drugs. The switching from IgG1 -> IgG4 producing immune cells should pose a serious issue in this regard, no?

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Levels will decline with time but then increase when reboosted or infected. They found the same pathogenicity when used in vivo & vitro, but if the recipient is not class switched they should decline again.

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Mar 29, 2023Liked by DoorlessCarpšŸ­

Yes, pregnancy is a sacred cow in my mind, having been born with birth defect.

I am also looking at the scientific articles confirming efficacy; I really appreciate what you share!

The Biochemical and Biophysical pathways/cascades continue to captivate my interest.

It would be interesting to cross reference our TCM libraries and compare notes at some point...

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Certainly would! I just don't have enough hours in the day to read everything I would like to from cover to cover.

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I think one of the most scary facts about the IgG4 switch is the fact that some of the interactions where IgG4 is involved are ANTIGEN-INDEPENDENT! So even if you had your three or even four LNP/mRNA vaccine shoots it is not just that these IgG4s are roaming your body looking for spike, any tumor out there will be happy to utilize those IgG4s to mask themselves and shut off parts of the immune system. And this takes the wind out of the sails of those that still think that the immune response to the vaccine would be limited to the antigens presented by the virus or the spike.

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Yes, some of the side chains aren't monovalent, which is partly why the PD1 inhibiting antibodies can cause myocarditis and other off target effects.

They can also persist via memory B cells for at least 8 years.

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Then a B-Cell depleting therapy like Rituximab or one of the other anti-CD20 bioequivalents used in MS (Ofatumumab, Ocrelizumab, etc.) should help rectify the issue, or would the non-cd20 expressing plasma cells present an issue?

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Well it kind of works for a while, but the side effects may be worse than the disease and it can leave you vulnerable to infections.

B-Cell depletion therapy in IgG4-related disease: State of the art and future perspectives

https://academic.oup.com/mr/article/33/2/258/6671753?login=false

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Jul 24, 2023Liked by DoorlessCarpšŸ­

Currently taking Ofatumumab(Kesimpta) due to MS diagnosis after COVID infection. Hopefully Kesimpta might cleanse my soul of the vaccine(partly at least, lol). Double jabbed, worked covid ward during delta. Saw crazy neurological symptoms in unvaccinated patients, people dying, sent multiple young patients to ICU... hence, me getting vaccinated(if I knew then what I knew now...)

First loading dose of kesimpta was rough, but since then it has been fairly smooth sailing. No crazy infections yet, but I stay fairly isolated.

Correct me if I'm wrong, but from my research, we're all f*cked, either through transfection or infection. Those with more infections/transfections being worse off than those with less spike exposure.

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Btw you might find something in this.

Micro dosed psilocybin is another I haven't listed yet.

Therapeutics for Multiple Sclerosis and Peripheral Neuropathy

https://doorlesscarp953.substack.com/p/therapeutics-for-multiple-sclerosis

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Jul 29, 2023Liked by DoorlessCarpšŸ­

Funny you mention psilo. 5ht2a agonists have been a source of interest of mine for almost a decade. I have yet to find an equivalent for BDNF. Unfortunately, sourcing mushrooms or spores are nearly impossible in my country(Germany). I know the synthesis is like 4 steps and fairly easy, however, I do not have access to a lab.

I found your list of MS therapeutics quite interesting. Why have you chosen to focus on a disease like MS if I might ask(being a fairly rare disease)?

I found this thread quite interesting, including the use of hymecromone: https://www.reddit.com/r/MultipleSclerosis/comments/13ie03g/disappearing_lesions/. The animal model for EAE was quite surprising. I would love to run a study in humans.

Also the possible use of androgens is quite interesting. Many neurologists seem to be oblivious to hormone replacement. 40% of men with MS have a testosterone deficiency. A study showed significant reduction in brain atrophy for the men given T after one year with reduction in fatigue and cognitive disability. They also showed an increase in grey matter.

https://www.frontiersin.org/articles/10.3389/fneur.2021.766308/full

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Jul 25, 2023Ā·edited Jul 25, 2023Author

I had Omicron & brain fog last year, unvaxxed. Took NAC & Q10 at the time, no follow up symptoms.

Now I rotate around hemp tea, quercetin, pomegranate extract, oolong tea, gynostemma tea, berberine extract, K2, zinc, C, glucosamine.

I have Echinacea, baicalin (advice: get some tincture, check my review) & Artemisia annua tinctures on standby for infections. Take when you get the first symptoms.

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Jul 29, 2023Liked by DoorlessCarpšŸ­

Why no Paxlovid? I have been wondering what to do about infections that are contained to the mucosal compartment. I surmise the majority of infections(mostly of the asymptomatic kind) are contained to the nasopharyngeal mucosal compartment. Things like Enovid look interesting but lack data. I wonder what effect will these infections have in terms of exposure to spike protein, and how do we keep them at bay.

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I 'liked' your reply, but I fucking dislike what is going on. Especially the percentage of the worlds population facing these issues. I am the only member of my family who did never get any of those LNP/mRNA drugs and I tried to warn everyone in my family including my wife since the start of the "vaccination" program and no one would listen.

Even now that I have Pfizer documents and an seemingly endless amount of scientific data that shows all the bad stuff that will happen to you when you get jabbed with this shit, people will not listen or read any of it.

I do hope Carp, that you are in contact with lots of others that are looking at this with their eyes open and some that reach larger audiences. There is no waiting for CNN or Spiegel or Guardian or Reuters to pick this up and spread it. This has to be done by us, the members of the public.

Thank you again Carp for what you do.

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Thanks Ghost, I don't have a huge reach but like you I was posting like crazy to Twitter & Facebook from 2020 with papers & graphs and was getting nowhere fast. In fact I got blocked, muted or argued with most of the time, even when it was posts about health committees advising against it for under 60s in many countries, before they got side swiped.

I thought maybe a third would buy the story and get juiced, but in the end everyone in my circle took as many doses as possible, and still are. They still think reinfections are ok and it would be worse if unjuiced. We cannot save members of a cult, effectively.

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This is horrible. If I understand it correctly, evertime they now get reinfected, their reprogrammed immune system will produce more and more IgG4 vs IgG1, raising their chance of getting cancer or autoimmune disease. None of those "juiced" people should be allowed to be bone marrow donors or organ donors or blood donors. Apart from the fact that the entire human population is facing years of crippling losses. This is getting worse and worse and worse. Have you tried reaching out to people like DR John Campbell or Matt Taibbi or Russel Brand? Basically anyone who is becoming more and more aware that there is something completely going wrong? There is also an Australian Senator who is getting vocal and a handful of British MPs that are starting to speak out.

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Mar 29, 2023Ā·edited Mar 29, 2023Author

I've certainly tried reaching out but no response is normally the response!

Mass screenings are warranted for cancers, heart conditions and T cell counts but it can be expensive, invasive and then you join a waiting list.

Another question is about recovery of the immune system, how much and over what timescale? Recovery from this sort of damage, comparable to radiotherapy, can take 1- 10+ years.

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Mar 29, 2023Liked by DoorlessCarpšŸ­

Gratitude for your scholarly works and the research papers from other independent thinkers with solid scientific backgrounds as well.

Also, the articles on therapeutics coincide with my garden plans, which are now requiring vertical elements to optimize space for more growing space for beneficial plants. The scientific research articles on therapeutics also stimulate further thought in my mind, regarding herbs and biochemical interactions in many ways. I am still learning...Thanks to people like you!

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Thank you Andrea. I need to learn more about herbal extraction & prep once I've grown the raw materials, and I plan to write an introduction to traditional Chinese medicine, although that's probably a life's work to learn!

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Mar 29, 2023Liked by DoorlessCarpšŸ­

I am on that path as well; my focus is on learning as much as I can during the tyrannical stages and then helping survivors when people are ready- w/prime directive of "first, do no harm". No pHARMa pills for me; as I had bad reactions to the only ones I took...so I'm on my own now.

I also study Chinese Medicine as well as Herbal/Nature methods, and Qigong from a Grandmaster from China/many other techniques...shared interest?

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Mar 29, 2023Ā·edited Mar 29, 2023Author

I have a library of TCM books and I dip into them for dosing guidance and need to know more about a practice going back thousands of years.

Chinese researchers are turning out hundreds of papers now which confirm their efficacy, explore the pathways and suggest modern applications.

Lack of safe Western treatments for hypertension in or after pregnancy in the west is unforgivable and little advanced from the Victorian era, for example.

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Mar 29, 2023Liked by DoorlessCarpšŸ­

I remember back in early 2021 I read a study on the quaxxine causing vaxx induced immune suppression, they called it VAIDS. I repeatedly spread that info far and wide and it is good to have new material to add to the VAIDS file. It was also called a "Crazy Conspiracy Theory" as well as Mis/Dis information and I was also repeatedly chastised by Big Tech over my sharing of the info. I firmly believe that the proof is in the pudding and that EVERY one of these Conspiracy Theories will, in the end, prove factual even to the dullard masses. Truly strange, interesting and dangerous times indeed !!

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Mar 29, 2023Ā·edited Mar 29, 2023Author

Indeed, a lot of us have been reading and posting & warning about the potential pathologies for 2-3 years, really hoping we were wrong and these would be edge cases. For CJD at least that appears to be the case, but for other conditions hold on to your hats!

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Mar 30, 2023Liked by DoorlessCarpšŸ­

Non-variant CJD can take years to show up. I don't know that we're out of the woods yet.

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Mar 30, 2023Ā·edited Mar 30, 2023Author

This could even play out over generations if the germline has been reverse transcripted. LNPs concentrate in the ovaries so it's not impossible, but may be recessive.

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Depressing thought, but yes - quite possible.

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Mar 29, 2023Liked by DoorlessCarpšŸ­

Can Of Worms comes to mind in more ways than one. With all the ailments cropping up slowly, I wonder if there is a timer ? Will they all suddenly spring forth with ferocity just like the comical spring loaded Can O Worms ? And when will that be ? The outlook does seem grim.

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Minimum latency periods for several cancers such as lymphomas and autoimmune disorders are around 2-5 years. But this will need 10+ years for accelerated heart disease to fully play out. We are talking decades to get the full picture.

Frustrating thing is that a lot of these can be controlled or slowed if caught early enough & treated and you stop your exposure to the agent.

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Mar 29, 2023Liked by DoorlessCarpšŸ­

Somehow, I don't see it taking near that long. With clots, myo-peri heart stoppage issues, cancers and autoimmune issues more like instant to 2 years with full on crisis within 5yrs.

With Round 2 already on the way, ...... they seem impatient !!

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Mar 29, 2023Ā·edited Mar 29, 2023Liked by DoorlessCarpšŸ­

And the time frame you are pointing out will exactly be the reason why most will not associate their condition with their jabs. The question I have is how can one figure out the underlying cause of their condition, if the consequences are that delayed?

Apart from people like you who diligently pull together the research that clearly lays out all the warning signs. I have a friend who is a family practitioner. I am sure he does not even know that the FDA tried to withhold the Pfizer documents for 75 years. Much less did he read study #185350 that shows that the IM injection ends up everywhere.

As far as I have been able to research, there is no scientific study out there to determine which cells in your body will get transfected and produce spike and which will not.

Would be interesting to know whether neurons can be transfected for instance and then be targeted by your immune system...

if that were the case anyone who got jabbed can now claim that he does not understand any of this due to brain damage he suffered from the vaccine.

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I investigated the pathophys of brain fog and everything pointed to endocytosis of S1 after crossing the BBB, with the gp120 inserts being responsible for neurotoxicity. Key thing is that it absolutely does not have to cause apoptosis but disrupts functionality eg dendrite growth. Compare with HAND you get the idea, it's not just glial activation. And they later found microtubule ferrying of virus components eg S1 between neurones.

https://doorlesscarp953.substack.com/p/pathophysiology-of-spike-protein

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Mar 31, 2023Ā·edited Mar 31, 2023Liked by DoorlessCarpšŸ­

https://www.jneurosci.org/content/30/18/6171

table 1 "Summary of advantages and disadvantages of different techniques commonly used to transfect mammalian neurons"

Specifically 'Lipofection'

https://de.wikipedia.org/wiki/Lipofektion

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But that is for the actual virus, right? Have you actually come across any study done on transfection of neurons in the brain by untargeted LNPs? If not, from what I have been able to find and understand, LNPs will transfect neurons. Neurons will produce spike protein and present it on their cell membrane. That makes them a target for NK cells which will in turn tell those neurons to kill themselves to contain a possible viral infection.

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Mar 29, 2023Liked by DoorlessCarpšŸ­

What a great work šŸ™

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Mar 29, 2023Liked by DoorlessCarpšŸ­

Posted it on several Twitter threads . Excellent ..šŸ™

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Thanks for sharing!

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Mar 29, 2023Liked by DoorlessCarpšŸ­

Your work is outstanding šŸ™

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Mar 29, 2023Ā·edited Mar 29, 2023Liked by DoorlessCarpšŸ­

Thank you. Now i have much less anxiety about a nuclear war. At least that would be a quick end of human civilization. It seems the mRNA vaccination program will bring a much slower collapse of human civilization. And i thought the idea, that even brain cells may become transfected and I will just be living in a future filled with brain damaged idiots, was bad. Now I know it is much darker than that.

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